|
protein
chaperones and stress response in Saccharomyces cerevisiae
The heat shock response
is highly conserved in all kingdoms, making it one of the most ancient
cellular regulatory systems. We have two primary interests, 1) how
heat shock is sensed and transduced to yield a genome-wide transcriptional
response, and 2) how protein chaperones, including the Hsp70, Hsp90
and the Hsp110 groups, function collaboratively within the cell
during normal growth and during adaptation to environmental stress.
The
baker’s yeast, Saccharomyces cerevisiae, is an ideal
microbial model system in which to investigate these questions,
due to its facile genetics, genomics and ease of manipulation. These
studies will directly impact our understanding of how human cells
respond to pathophysiological states such as cancer and anoxia which
strongly induce a heat shock response. In addition, there is growing
evidence that the amyloid diseases of protein misfolding, including
prion-based maladies such as Creutzfelt-Jakob (mad cow), and other
triplet-repeat type diseases including Alzheimer's, Parkinson's,
and Hunginton's, are intimately linked to protein chaperone expression
and function.
SELECTED
PUBLICATIONS:
Shaner
L, Wegele H, Buchner J, and Morano KA (2005) The yeast Hsp110 Sse1
functionally interacts with the Hsp70 chaperones Ssa and Ssb. J
Biol Chem, 280, 41262-41269. [abstract]
Trott,
A., Shaner, L. and Morano, K.A., (2005) The molecular chaperone
Sse1 and the growth control protein kinase Sch9 collaborate to regulate
protein kinase A activity in Saccharomyces cerevisiae. Genetics,
170:1009-1021.[abstract]
Trott
A and Morano KA (2004) SYM1 is the stress-induced Saccharomyces
cerevisiae ortholog of the mammalian kidney disease gene Mpv17
and is required for ethanol metabolism and tolerance during heat
shock. Euk Cell, 3: 620-631.[abstract]
Shaner
L, Trott A, Goeckeler JL, Brodsky, JL, and Morano KA (2004) The
function of the yeast molecular chaperone Sse1 is mechanistically
distinct from the closely related hsp70 family. J Biol Chem 279:
21992-22001.[abstract]
Trott
A and Morano KA (2002) The yeast heat shock response, in Yeast Stress
Responses, eds. Stefan Hohmann and Willem Mager. Springer-Verlag:
Heidelberg, pp. 71-119.
[compete
list of publications on PubMed]
|
