These maps represent our current working model of the order of the DOE contigs around the two chromosomes. The black horizontal lines represent, to scale, the size of the contigs. Above each black line is the contig number. Small contigs have their numbers written vertically, e.g., the second contig on the chromosome I map is 62. Contigs that wrap onto a second line have ellipsis (....) next to the contig number. The contig number can be used to download DNA sequences from here and annotation information for that contig from here.
Sequence gaps between DOE contigs are represented by orange or blue circles. Orange circles indicate gaps that we have successfully closed by PCR followed by sequencing of the PCR products. Blue circles indicate gaps that have been recalcitrant to this closure strategy.
A brace { at the top left of each chromosome map indicates which lines of the map should be read together e.g., the top left line of the chromosome I has contigs 78, 62, 162, 102, 138, 48, 42 and 121 with the gene cerI placed on contig 121. At the right end of the top line is the start of contig 166 and the photosynthesis gene cluster. Note that both of these wraps onto the next line.
Some pulse field gel mapped and sequenced marker genes have been placed above the contigs in red boxes. Note that the size of the box does not represent the size of the gene, it simply indicates that, for example, trpA maps to contig 180.
On chromosome II there is light green line beneath the DOE contig lines. This indicates the contigs that were generated by amalgamating the data from the University of Texas CII specific project and the DOE whole genome project.
Note that the size of chromosome I is larger than on previously published
maps, 3.11 vs 2.97 Mb. The size may increase (or decrease) as assembly
continues. At the current level the difference is <10% of previously
published maps. Chromosome II came out to be close to the previously published
0.9 Mb. We estimate that the sum of the gaps in the DOE CII sequence is
11 kb.